To further advance the understanding of epigenetic regulation in farmed fish and for screening epigenetic interventions with putative positive effects on growth and other desired traits, appropriate experimental models are needed. While studies in live fish are unique to ultimately evaluate growth rate and other responses, in vitro models are suited to screening and mechanistic studies.

The results of the study on viability, structure, proliferation, and functionality of explants support their use in short-term assays. The explants responded to epigenetic manipulations by changing the expression and activity of some endogenous epigenetic regulators. Moreover, the study revealed that some key players of the growth axis in this fish species are directly or indirectly regulated by epigenetic mechanisms such as DNA methylation. These explants are already being used at ICMAN-CSIC for epigenetic research at the genome level in gilthead seabream and results are on their way.

This research was funded by the project BreamEpiMap (Grant: PID 2021-128540OAI00) funded by MCIN/AEI/10.13039/501100011033 and by “ERDF A way of making Europe”, and by project epiMODEL2 (ref 202240I011, CSIC).

Full study available at:
https://www.frontiersin.org/journals/physiology/articles/10.3389/fphys.2024.1410660/full

Citation: Perera E, Román-Padilla J, Hidalgo-Pérez JA, Huesa-Cerdán R, Yúfera M, Mancera JM, Martos-Sitcha JA, Martínez-Rodríguez G, Ortiz-Delgado JB, Navarro-Guillén C and Rodriguez-Casariego JA (2024), Tissue explants as tools for studying the epigenetic modulation of the GH-IGF-I axis in farmed fish. Front. Physiol. 15:1410660. doi: 10.3389/fphys.2024.1410660

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